NANOPLEX™ biomarker detection
SERS Imaging Applications
NANOPLEX™ biotags can be used potentially in any application where fluorescence labelling and conventional stains have traditionally been used, for example, for biological imaging. In both ex vivo and in vitro imaging applications, NANOPLEX™ biotags bring the same performance benefits that they offer to in vitro diagnostics. Namely, the ability to obtain signal against a background of sample autofluorescence, improved analyte detection sensitivity and extended multiplex capabilities.
Quantitative ImmunoHistochemical labellin:
- Unaffected by background from classical staining.
- Transformation of IHC to quantitative protocol.
- Demonstrated multiplex of up to four analytes in tissue samples.
Cellular labelling:
- Cell surface labelling capabilities with standard NANOPLEX™ biotags.
- Smaller tags offer improved cellular penetration
- Binnig of Raman spectral peaks (1600 cm-1 in example) allows rapid imaging and multiplex detection.
- No photobleaching of signal on extended exposure.
Brightfield Reflectance Image
Integrated Raman Image
1500 cm-1
1532 cm-1
1600 cm-1
1635 cm-1
