NANOPLEX™ No-wash Assay
NANOPLEX™ Direct is a homogeneous assay format rivalling conventional ELISAs while offering several advantages including
- Simple procedure using a one-step, no-wash assay format
- High sensitivity, on par with heterogeneous, amplified systems
- Leverages the performance of surface-enhanced Raman scattering (SERS)
- Near IR detection allows for data collection in whole blood, serum and plasma backgrounds
- Multiplexing capability of the technology allows for quantitation of multiple analytes in a single reaction
- Benchtop reader and proprietary software for data analysis
Procedure
Advantages of NANOPLEX™ Direct
Detection of TNF-a comparing ELISA and NANOPLEX™ Direct workflow
| ELISA* | NANOPLEX™ DIRECT | |
|---|---|---|
| Total Assay Time | 5 hours | < 1 hour |
| Number of wash steps | 3 | 0 |
| Amount of TNF-a antibody Capture Detection |
400 ng/well 7 ng/well |
450 ng/tube 8 ng/tube |
| Detection Limit | 2 pg/mL | 3 pg/mL |
Precision in Biological Samples
Example of cTnI and CKMB detection in serum samples (Bio-Rad Liquicheck Cardiac Markers Plus Controls) in a multiplexed assay
| COMMERICAL ANALYZER* |
NANOPLEX™ DIRECT |
COMMERICAL ANALYZER* |
NANOPLEX™ DIRECT |
|
|---|---|---|---|---|
| BioRad Controls | cTnI Clinical Analyzer Range ng/ml |
cTnI 2-plex NANOPLEX™ Direct ng/ml(**) |
CKMB Clinical Analyzer Range ng/ml |
CKMB 2-plex NANOPLEX™ Direct ng/ml(**) |
| Level 1 | 0.4 – 2.2 | 2 (14) | 2 – 5 | 4 (8) |
| Level 2 | 3 – 20 | 13 (8) | 10 – 18 | 17 (14) |
| Level 3 | 9 – 50 | 28 (4) | 27 -81 | 40 (12) |
* Values determined on 20 commercial clinical analyzers
** CV %, 6 repetitions
Multiplexed Technology
- Quantitation of multiple analytes in a single reaction
- Assays perform independently of each other
